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Oh, you think that, do you??
Are you a medical professional or a scientist?
Are you involved in the care of an individual trans person, or are they participating in your research?
Since you've answered no to all of those, and even if you had answered yes, then still no, in almost all cases, the distinction isn't relevant, and more importantly, is none of your fucking business.
And since I doubt you've had your own chromosomal make up tested, or even your hormone levels, you probably couldn't commit 100% to your own (or anyone else's) fucking "biological sex" so questioning that of others (or worse, demanding proof) only because they're trans isn't only intrusive, perverted, and outright transphobic, it's also entirely unscientific (the term as well as the concept/construct), and as mentioned above - none of your motherfucking business.
So you can take your generic "I'm not a transphobe but...bIoLoGiCaL sEx" excuse and shove it, and if being called a transphobe bothers you more than being one, perhaps try educating yourself instead of continuing to regurgitate the most commonly used transphobic talking points in existence..
You seem like you want to be mad at something. I have no problem referring to you as whatever pronouns, names, sex, gender, etc. It's just that we need words to describe chromosomal differences in biology and sex is what we chose to do that. Just in scientific fields. I am not saying that anyone's personal sex identity is my business, but it is relevant in scientific and medical studies. Those are everybody's business.